Tube for Drawing Blood for a Blood Test

PHLEBOTOMY SERVICES

EVACUATED BLOOD COLLECTION TUBE GUIDE.
Listed in Order of Draw for Blood Collection Tubes and Syringe Collections.

STOPPER COLOR	CONTENTS	VOL.	USES/COMMENTS
Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. .
Royal Blue	No additive (serum); special glass and stopper material	7.0 mL	Most drug levels, toxicology screens, and trace elements
Red	No additive	7.0 mL	Cryoglobulins and CH₅₀
Light Blue	3.2% Sodium Citrate	4.5 mL	PT, PTT, TCT, CMV buffy coat, Factor Activity Tube MUST be filled 100% - No exceptions!
Gold Top (Serum Separator, "SST")	Contains separating gel and clot activator	6.0 mL	Most chemistry, endocrine and serology tests, including Hepatitis and HIV
Light Blue - Yellow Label on Tube	Thrombin	2.0 mL	For FDP test ONLY; Obtain tube from Core Lab Coag; Allow to Clot
Green	Sodium heparin(100 USP Units)	5.0 mL	Ammonia, Lactate, HLA Typing
Tan	K² EDTA	5.0 mL	Lead levels
Yellow	ACD Solution A consists of trisodium citrate, citric acid and dextrose	8.5 mL	DNA Studies, HIV Cultures
Pink	(K₂)EDTA	Draws 6 mL - Minimum 4 mL	Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load
Pearl Top (Plasma Preparation, "PPT")	Separating gel and (K₂)EDTA	4.0 mL	Adenovirus PCR Toxoplasma PCR HHV-6 PCR
Lavender ("Purple")	(K₂)EDTA	3.0 mL	CBC/Diff/Retic/Sed Rate, FK506, Cyclosporin, Platelet Ab, Coombs, Flow Cytometry

NOTE: All tubes sterile.

Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first.

Blood Culture Options (Specify Culture Type when Ordering)

Culture Type	Patient Population	Test ID	Test #	BottleType/Comments
Culture, Blood, Routine	All Patients not Meeting Criteria Below NOTE: This culture will recover HACEK organisms with no change in collection or incubation conditions. If Brucella or Francisella are in the differential, extended incubation may be required. Please contact the Microbiology Lab (966-4056).	BROU	9500	BD BACTEC Plus Aerobic/F (Grey Cap/Blue Ring) (Optimal Volume 8-10 mL) Lawson# 052346(UOM box of 50 bottles) BD BACTEC Anaerobic Lytic/10 (Purple Cap/Maroon Ring) (Optimal Volume 8-10 mL) Lawson# 052347 (UOM box of 50 bottles)
Culture, Blood, AFB	HIV+ patients with CD₄ Count <100; Suspected MAC infections	AFBB	9684	BD BACTEC Myco/F Lytic (White Cap/Red Ring) Available from Phlebotomy Lab (Optimal Volume 1-5 mL)
Culture, Blood, Pediatric	Patients Weighing <40 kg	BPED	9548	BD BACTEC Plus aerobic/F (Grey Cap/Blue Ring) (Optimal Bolume 8-10 mL) For Patients <20 Kg, 1-4 mL is acceptable For Patients 20-40 Kg, 4-8 mL in one bottle. For Patients >40 Kg (See Routine above)
Blood Culture Mould	Suspected infections due to dimorphic (endemic) fungi, Fusarium sp., Trichosporon sp., Malassezia furfur (Specify at Collection).	BPATH	9507	Isolator Yellow Top (10 mL Volume) Available from Plebotomy BD BACTEC Plus Aerobic/F (Grey Cap/Blue Ring) (Optimal Volume 8-10 mL) Lawson# 052346 (UOM box of 50 bottles) BD BACTEC Myco/F Lytic (White Cap/Red Ring) Available from Phlebotomy Lab (Optimal Volume 1-5 mL)